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The bicistronic expression vector pDgD/HER2pr gD IRES TK contains the human HER 2/neu promoter and the coding regions of gD and HSV TK linked by an IRES sequence, in addition to 5' and 3' flanking regions encompassing gG and gI, respectively, timberland pas cher femme to provide homology regions for homologous recombination with the CW1 virus. MBT 2 cells were transfected with pDgD/HER2pr gD IRES TK plasmid followed by infection with CW1 virus, yielding YP2 virus. Restriction enzyme cleavage sites shown are BamHI (B), HindIII (H), NotI (N), EcoRI (R), and XhoI (X).tyy9.17tyy9.17


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