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Top of pageResults and DiscussionGA reduces HER 2/neu protein level and increases the expression of MHC class I and HSP70 in vivoTo examine the dose required for degradation of p185neu in vivo, established murine bladder tumor (MBT) louboutin outlet uk 2 bladder tumors were treated with escalating concentrations of GA intratumorally, and the expression of p185neu was examined by Western blot analysis. At concentrations of 2.5 g and higher, GA inhibited the expression of p185neu for up to 5 days (Figure 1a). P185neu is known to downregulate prada outlet the expression of MHC class I.34, 35 MHC class I presentation on MBT 2 tumor cells was upregulated by GA treatment as shown by flow cytometric analysis (Figure 1b).
40644 Notice In hereby given that Albert C. Vietzcn. Sr whose Post Office address Is 1021 Foster Avenue. Deletion cheap prada bags of gE reduces virulence,13 because gE deleted PrV has a reduced capability to infect second and third order neurons of the olfactory and trigeminal routes in the nervous system.14, 15 The complex formed by gE and gI is one of the prime determinants for neuroinvasion and neurovirulence. PrV longchamp soldes encoded thymidine kinase (TK) supports viral DNA replication and plays an important role in virulence and neuroinvasiveness.16, 17 Moreover, it is required for production of infectious virions in non dividing cells.18 Genetically modified herpes simplex virus (HSV) mutants christian louboutin soldes are effective oncolytic viruses targeting rapidly dividing cells in animal models,19, 20 and have completed Phase I clinical trials in treating recurrent high grade glioma.21, 22 PrV can infect and destroy both mouse and human tumors in vivo without signs of disease, suggesting its potential moncler outlet use as an oncolytic agent for human tumors.23 Given that deletion of virulence genes of PrV is necessary for safety concerns, but also renders PrV less able to replicate and thereby less cytolytic or oncolytic, in this study we generated YP2 virus, a gE/TK defective PrV mutant carrying zanotti pas cher both gD and HSV 1 TK genes under the transcriptional control of the HER 2/neu promoter. Replication and spread of YP2 would be expected to be restricted due to lack of gD and TK expression in normal cells or cancer cells expressing low or negligible levels of HER 2/neu.zy0919


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