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The PCR products were verified by automated DNA sequencing (data not shown). To confirm the genomic structure of YP2, virion DNA was cleaved with BamHI, electrophoresed, transferred to a nylon membrane, and hybridized louboutin pas cher femme to a radiolabeled gD specific probe. In TNL, the gD gene resides within the 7.4 kb BamHI fragment of the PrV genome.24 CW1 was derived from TNL, in which the gD gene was replaced by the HSV TK gene.25 The HSV TK expression cassette in CW1 was replaced with the HER 2/neu promoter gD IRES HSV TK fragment to generate YP2.JXM.9.22


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