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A conserved aspartic acid in the second MBT repeat of 3xMBT cheap jordans for sale mediates recognition of both H3K9me1 and H4K20me1 peptidesThe MBT repeats each contain a ligand binding pocket lined by aromatic residues (Wang et al., 2003) and a conserved aspartic acid (D248, D355 and D459). As a D215N mutation in the Drosophila sex comb on midleg protein results in developmental defects (Bornemann et al., 1998), we generated GST knock off michael kors 3xMBT constructs with D (aspartic acid) to N (asparagine) substitutions in each of the MBT repeats. A single mutation (D355N) in the second MBT repeat abolished binding to both H4K20me1 and H3K9me1 peptides, whereas the corresponding mutations in the first and third repeats did not perturb the 3xMBT interaction (Figures 2c and d).tyy9.26


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