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Using similar techniques, we determined that the 3xMBT repeats by themselves are capable of chromatin association (Supplementary Figures S1A, B, and C). Transiently expressed full length L3MBTL1 or the 3xMBT repeat region, both localized to chromatin (in 293T cells) whereas the N terminus of L3MBTL1 did not (despite good nuclear mulberry outlet accumulation; Supplementary Figure S1B). A deletion mutant of L3MBTL1 lacking the 3xMBT region (L3MBTL1 did localize to the chromatin fraction.
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