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In moncler tracksuit Xenopus embryos, cell cycle elongation and degradation of Cdc25A (a Cdk2 Tyr15 phosphatase) occur naturally at the midblastula transition (MBT), at which time a physiological DNA replication checkpoint is thought to be activated by the exponentially increased nucleocytoplasmic ratio. Here we show that the checkpoint kinase cheap moncler jackets Chk1, but not Cds1 (Chk2), is activated transiently at the MBT in a maternal/zygotic gene productregulated manner and is essential for cell cycle elongation and Cdc25A degradation at this transition. A constitutively active form of Chk1 can phosphorylate Cdc25A in vitro and can target it rapidly for degradation in preMBT embryos.qq1008


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