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Top of pageResultsThe 3xMBT region of L3MBTL1 is sufficient for its chromatin association in vivo and in vitroTo test whether L3MBTL1 is chromatin associated in vivo, we isolated nuclei from K562 erythroleukemia cells that express appreciable levels of L3MBTL1 (MacGrogan et al., 2004), and generated soluble moncler outlet uk (nucleoplasm) and insoluble (chromatin) fractions, as described (Mendez and Stillman, 2000). L3MBTL1 protein is found in the chromatin fraction (residual chromatin pellet; lane 1, Figure 1b). Digestion of the pellet with increasing concentrations of micrococcal nuclease (MNase) releases L3MBTL1 (and the TATA box binding protein new balance 574 pas cher (TBP)) into the MNase buffer supernatant, identifying L3MBTL1 as a chromatin associated factor.
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