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To define the basis for the chromatin association of the 3xMBT repeats, we performed in vitro pull down assays using recombinant 3xMBT repeats fused to a GST tag (GST 3xMBT) and calf thymus histones (Figure 1c). To exclude the possibility that the simultaneous binding to H3 and H4 is a consequence sac longchamps pas cher of their hetero oligomerization, acid extracted histones H2A, H2B, H3 and H4 were individually purified from unsynchronized HeLa cells. In contrast, no interaction was detected ...
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