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Top of pageResultsThe 3xMBT region of L3MBTL1 is sufficient for its chromatin association in vivo and in vitroTo test whether L3MBTL1 is chromatin associated in vivo, we isolated nuclei from K562 erythroleukemia cells that express appreciable levels of L3MBTL1 (MacGrogan et al., 2004), and mulberry outlet generated soluble (nucleoplasm) and insoluble (chromatin) fractions, as described (Mendez and Stillman, 2000). L3MBTL1 protein is found in the chromatin fraction (residual chromatin pellet; lane ...
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Hence, IMAGE clones 42082,1676403, and 490234 containing portions of human l(3)mbt (h l(3)mbt), CGI 53, and MYBL2 genes, respectively, mapped by Southern analysis to a region that contains several contiguous PACs that overlap with clone dJ1118N11 (Table 3). IMAGE clones 79307 and 41332, which contain portions louboutin shoes outlet of the sts T63166 and TNRC9/CAGF9 genes, respectively, were mapped to several PAC clones that encompass clone dJ440H8.A second approach involved exon trapping, using ...