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To assess whether the 3xMBT domain binds H4K20me1 in a transcriptionally repressive or activating context, we immunoblotted the HeLa histone H4 species pulled down by GST 3xMBT with a panel of modification specific antibodies. H4 species recognized by GST 3xMBT were devoid of the acetylation marks H4K5 and H4K16 prada outlet online (but enriched for H4K20me1). HeLa histone H3 recognized by GST 3xMBT lacked H3K9 acetylation (Supplementary Figure S4), supporting the idea that L3MBTL1 may be more commonly ...

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