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To examine the expression and subcellular localization of the h l(3)mbt proteins, we raised rat polyconal antiserum against the GST h l(3)mbt I fusion proteins. First, a Western blot analysis of VA13 cells, which were transiently transfected by the Myc tagged h l(3)mbt mulberry outlet york I expression plasmid (pBJ Myc h l(3)mbt I) or Myc tagged h l(3)mbt II expression plasmid (pBJ Myc h l(3)mbt II), and U2OS osteosarcoma cells, which are known to express high levels of PcG proteins (Alkema et al., 1997a,b), was performed using the anti h l(3)mbt antibody. Approximately 105 kDa bands corresponding to overexpressed cheap timberland boots h l(3)mbt I or II proteins were detected from the lysates of pBJ Myc h l(3)mbt I and II transfected VA13 cells with the anti Myc antibody (Figure 3a, lanes 2 and 3) and the anti h l(3)mbt antibody (Figure 3a, lanes 6 and 7).
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